Enzymes for pet food digest production
Pet Food Digest is meat that has been broken down to be used for pet food flavoring. A model system for the production of digest includes finely grinding the meat product or other protein. Water may be needed depending on whether the meat product is frozen and needs to be thawed or if it is fresh. Add the enzyme of choice at a rate of 0.1% to 0.25% based on the total weight of the meat. Addition of the enzyme may be done at the start of the cook cycle or when the slurry reaches the desired temperature. The enzyme is deactivated by raising the temperature of the slurry to the enzymes deactivation temperature and holding for 10 minutes. Papain is arguably the most popular enzyme for digest production, however other enzymes may be more effective for certain types of byproducts or may give a different palatability result.
Papain is a broad spectrum protease that is active over a large pH and temperature range. Papain is active on a variety of substrates such as liver, lung, or other viscera. It is a very heat stable protease and can easily tolerate reaction temperatures in the range of 140-160 degrees F. Papain can be inactivated by holding at 185 – 190 degrees F for 10 minutes. These conditions may vary depending on other processing parameters.
Papain activity assays: Milk Clot Unit (MCU) • FCC Botanical Protease Units (FCC-PU) • Tyrosine Assay Method (TU)
EDC is the only producer of Papain in the western hemisphere. At our factory in Scranton, Pennsylvania, we produce standardized Papain powders and liquids, as well as unstandardized concentrates. We also produce a no sulfite added Papain that is OMRI Listed for use in Organic food processing applications. EDC’s Papain products include:
ENZECO® PURIFIED PAPAIN CONCENTRATE
A concentrated papain powder.
ENZECO® PURIFIED PAPAIN RS CONCENTRATE
Papain that is processed without the use of sulfites. The papaya latex in this product has been processed without the addition of sodium metabisulfite. This results in papain with substantially reduced levels of SO2.
This product contains only the botanical enzymes and has no other ingredients. It has earned an OMRI listed® certification, which means that it may be used in certified organic operations or food processing under the USDA National Organic Program.
ENZECO® PURIFIED PAPAIN RS 600
EDC’s no added sulfite papain standardized to 600 TU/mg on maltodextrin.
Liquid papain, standardized to activities of 100 and 200TU/mg.
LIQUIPANOL® RS 100 and RS 400
Liquid papain that is processed without the use of sulfites Liquid papain, standardized to activities of 100 and 400TU/mg. These products have earned OMRI listed® certifications, which means that it may be used in certified organic operations or food processing under the USDA National Organic Program.
ENZECO® BACTERIAL PROTEASE BL
A protease enzyme preparation derived from a non-GMO stain Bacillus licheniformis. It contains endopeptidase and exopeptidase, which are used for hydrolyzing proteins and for improves flavor.
Activity assays: Protease Assay (PC)
MULTIZYME® NEUTRAL BACTERIAL PROTEASE 500
This is a neutral protease derived from Bacillus licheniformis. (formally B. subtilis) that has optimum activity from pH 5.5-8 and contains no/very low amylase side activity.
Activity assays: Protease Assay (PC)
ENZECO® PROTEASE FNP
A fungal neutral protease preparation derived from a non-GMO strain of Aspergillus oryzae that has optimum activity from pH 5-7 and contains a high level of aminopeptidase activity and acid carboxypeptidase. It also contains a high level of debittering enzymes (Leucine aminopeptidase (LAP) and carboxypeptidase) and endo-action proteases for flavor enhanced hydrolyzed proteins.
Activity assays: Casein Folin A (CFA), Activity can also be reported on Hemoglobin Units on Tyrosine basis (HUT)
ENZECO® ALKALINE PROTEASE L - 660
An alkaline protease derived from a non-GMO strain Bacillus licheniformis. It is an endopeptidase used for hydrolyzing proteins. It also contains amylase, lipase and other side activities.
Activity assays: Detergent Alkaline Protease Unit (DAPU)